ATP synthase is a promising target for identifying activated and non-activated adipose tissues.
basic_science · Level V
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- Record sourced from PubMed, PMID 41986300.
- Also identified by DOI 10.1038/s41467-026-71343-w.
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Abstract
Adipose tissue has gained increasing attention as a therapeutic target to combat human obesity and related metabolic disorders. We propose ATP synthase as a target to identify activated and non-activated adipose tissue. We investigated ATP synthase using the radiotracer [<sup>11</sup>C]J147 and confirmed the specificity of the radiotracer by in vitro autoradiography, cell knockdown studies, and in vivo competition binding studies. In addition to the interscapular brown adipose tissue (BAT), [<sup>11</sup>C]J147 could visualise several other BAT depots (supraspinal, infrascapular and axillary BAT) via in vivo positron emission tomography imaging after activation with a β<sub>3</sub>-adrenergic receptor agonist, as confirmed by immunohistochemistry and biodistribution studies. Furthermore, [<sup>11</sup>C]J147 demonstrated higher sensitivity for BAT and WAT (white adipose tissue) identification compared to the commonly used radiotracer [<sup>18</sup>F]FDG and the mitochondrial complex I tracer [<sup>18</sup>F]BCPP-EF. Our study uncovers ATP synthase as a promising target for monitoring adipose tissue and [<sup>11</sup>C]J147 could facilitate drug development for metabolic diseases such as obesity.