A series of spontaneously blinking dyes for super-resolution microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 41986687.
- Also identified by DOI 10.1038/s41592-026-03062-5 and PMC identifier 13167466.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Spontaneously blinking fluorophores toggle between nonfluorescent and fluorescent forms without caging groups or redox buffers, enabling super-resolution imaging. The intrinsic blinking of such dyes is governed by molecular structure and modulated by environment; there is no one-size-fits-all fluorophore suitable for every imaging context. We report dyes with tuned on:off ratios that enable single-molecule localization microscopy and super-resolution optical fluctuation imaging of biomolecular structures in vitro and in cells.
Medical subject headings
- Fluorescent Dyes
- Single Molecule Imaging