Selective on-chip DNA synthesis using electric field-assisted PCR.

Abstract

This study introduces a robust selective on-chip DNA synthesis platform utilizing an electric field-assisted polymerase chain reaction (E-PCR) system based on a microelectrode array. We demonstrate the use of electric field manipulation for the selective immobilization of DNA and enzyme-mediated single-stranded DNA synthesis, achieving precise spatial control at the microscale. By optimizing electric field patterns, we achieved high-efficiency synthesis and addressed critical challenges in electrochemical stability and DNA integrity. The E-PCR system enables selective DNA synthesis, length-controlled synthesis, and sensitive nucleic acid detection. Our findings provide a scalable foundation for high-throughput enzymatic synthesis, offering significant potential for DNA data storage, synthetic biology, and molecular diagnostics.