Population dynamics of generalist and specialist strategies under feast-famine cycles.

Abstract

Microbial populations exhibit a broad spectrum of nutrient utilization strategies, ranging from those utilizing diverse nutrients, called "generalists," to those highly adapted to specific nutrients, called "specialists." Identifying the conditions for the diversification of nutrient utilization strategies is one of the central questions in ecology. Previous theoretical studies have shown that trade-offs among different resource utilization functions in which cells cannot utilize broad types of substrates at nearly optimal efficiency are crucial for the emergence of diverse strategies. Additionally, in natural settings, nutrient availability often fluctuates over time, imposing another trade-off on the cells; cells that grow rapidly under nutrient-rich conditions tend to have a higher death rate under nutrient-poor conditions, leading to a growth-death trade-off. This additional trade-off can contribute to the emergence of diverse strategies. Here, we introduce a mathematical model that simultaneously incorporates the resource-use trade-off and the growth-death trade-off. Nutrient supply was modeled as discrete stochastic events, mimicking temporal changes in nutrient availability. We show that the phenotype with a higher ratio of growth rate to death rate dominates the population; that is, the strength of the growth-death trade-off plays a crucial role in the emergence of distinct strategies. We also found that a sparse and uncertain nutrient supply favors specialists, increasing their temporally averaged abundance. Our findings highlight the crucial role of temporal environmental variation and the resulting growth-death trade-off in driving diversification of microbial nutrient utilization strategies.