<i>Wolbachia</i>-mediated viral transmission enhancement in insect vectors.

Abstract

The white-backed planthopper (<i>Sogatella furcifera</i>) serves as the vector for the southern rice black-streaked dwarf virus (SRBSDV), with varying transmission efficiencies across populations. This research identifies <i>Wolbachia</i>, a common insect symbiont, as a key facilitator in breaching the salivary gland barrier for SRBSDV, revealing a mechanism by which the virus exploits an insect symbiont for transmission. Through field and laboratory investigations, it was observed that high-transmission (HT) planthopper populations contained high levels of <i>Wolbachia</i>, while low-transmission (LT) populations had minimal titers. Experiments involving thoracic injections confirmed <i>Wolbachia</i>'s specific role in infiltrating salivary glands, as SRBSDV was unable to colonize glands in <i>Wolbachia</i>-depleted insects despite being present systemically. Ultrastructural analysis showed <i>Wolbachia</i> enveloping SRBSDV particles within gland cells, further supported by molecular assays indicating a direct interaction between <i>Wolbachia</i> surface protein (WSP) and viral P8 capsid protein. Disruption of this interaction using anti-WSP antibodies reduced salivary gland viral load and transmission rates, underscoring its functional importance. These results contrast with <i>Wolbachia</i>'s antiviral effects in mosquitoes, highlighting a context-dependent "hitchhiking" strategy for viral dissemination. The WSP-P8 interaction presents a specific target for inhibiting SRBSDV transmission without resorting to pesticides, proposing a symbiont-informed approach as a sustainable strategy against rice viral diseases.