An encyclopedic regulatory and functional atlas of RNA interactomes.

Zhou, Keren; Huang, Junhong; Liu, Shurong; Zheng, Wujian; Liu, Shun; Xing, Yinghao; Cao, Yinan; Chen, Yibo et al. · Nat Methods · 2026

basic_science · Level V

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Abstract

RNA molecules interact extensively with each other and with RNA-binding proteins (RBPs) to regulate gene expression. To improve detection of RBP-RNA and RNA-RNA interactions, we developed the rbsSeeker and rriScan tools and integrated the interactions into the Encyclopedia of RNA Interactomes (ENCORI), providing web-based modules to explore RNA interactions. Using this resource, we identified and validated novel N<sup>6</sup>-methyladenosine (m<sup>6</sup>A)-associated proteins, an orphan small nucleolar RNA guiding rRNA pseudouridines and target-directed microRNA degradation events, establishing ENCORI as a framework for studying RNA interactomes.