Accurate quantification in proteomics with QuantUMS.
basic_science · Level V
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- Record sourced from PubMed, PMID 42204360.
- Also identified by DOI 10.1038/s41587-026-03131-2.
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Abstract
In mass-spectrometry-based proteomics it remains challenging to ensure the accuracy of protein quantities. Here we introduce QuantUMS (quantification using an uncertainty-minimizing solution), a machine learning-based method that dynamically tunes the quantification algorithm to minimize quantitative errors. When applied to data-independent acquisition proteomics, QuantUMS increases accuracy and precision, ameliorates ratio compression bias and enhances differential expression analysis. It further reports an uncertainty measure enabling quality control of individual quantities.