Bacterial contamination of surgical instruments in 25-gauge pars plana vitrectomy: Incidence and risk factors.

Miyako, Fumiya; Harada, Yosuke; Hiyama, Tomona; Kashiyama, Seiya; Kawamoto, Saori; Yokota, Chika; Minamoto, Akira; Ota, Hiroki et al. · PLoS One · 2026

retrospective_cohort · Level III

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Abstract

To evaluate the incidence of surgical instrument contamination associated with microincision vitrectomy surgery. Ninety-six eyes from 86 immunocompetent patients who underwent 25-gauge pars plana vitrectomy from December 2023 to February 2024 in our institution were retrospectively analyzed. Surgical instrument contamination was detected in 7 cases (7.3%). Bacterial species were isolated from the trocar, endoilluminator, and beveled probe. Postoperative endophthalmitis did not occur during the 2-month follow-up period. Use of preoperative antibacterial eye drops was significantly associated with lower risk of bacterial contamination. Contamination may occur 25-gauge pars plana vitrectomy, even when preoperative antibacterial eye drops are used in conjunction with povidone-iodine irrigation and adequate vitreous dissection.

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