Considerations and best practices for respirometry.

Ball, Andréa B; Stiles, Linsey; Divakaruni, Ajit S · Cell Metab · 2026

review · Level V

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Abstract

Oxygen consumption rate (OCR) measurements are now widely used to assess mitochondrial function and cellular metabolism. Here, we review the strengths and limitations of plate-based respirometry, propose unified reporting standards, and discuss how OCR measurements can be integrated with complementary assays to evaluate cellular energy demands and mitochondrial function.