Convergence of KRAS Mutations and MTAP Loss in Pancreatic Cancer: Genomic Landscape and Clinical Implications.

Braganca Xavier, Camila; Shah, Pooja A; Yakimova, Daria; Kravets, Andrey; Belousov, Dmitrii; Kudryavtseva, Ksenia; Massaro, Matteo; Paradiso, Francesca et al. · Clin Cancer Res · 2026

retrospective_cohort · Level III

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Abstract

Comprehensive genomic profiling has prognostic and predictive value for patients with pancreatic adenocarcinoma. We reviewed clinical and molecular data from 4,009 samples from patients who had undergone the BostonGene Tumor Portrait test between 28.10.2021 and 08.10.2024, and 2,181 samples from the BostonGene pancreatic adenocarcinoma meta-cohort, collected from various data hosts and processed by BostonGene automated pipelines. In this high-purity pancreatic adenocarcinoma cohort, 24% harbor homozygous MTAP deletion, and the co-occurrence of KRAS mutations and MTAP loss was common (18.9% of all pancreatic adenocarcinomas). This association identified a subgroup with worse survival outcomes. MTAP-deficient tumors harbor more fibrotic, less immune-enriched microenvironments and have shorter survival. Within the co-mutated tumors, the most frequently detected KRAS variants were G12D, G12V, and G12R, with the last one slightly more related to immune-enriched TME features than the other KRAS variants. Comprehensive genomic profiling is essential for patients with PAAD and carries both prognostic and predictive value. MTAP loss KRAS-mutant PAAD represents a subgroup of immune-excluded PAADs with a poor prognosis.