A minimal transcription factor network is sufficient to drive paclitaxel biosynthesis.
basic_science · Level V
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- Record sourced from PubMed, PMID 42247491.
- Also identified by DOI 10.1126/sciadv.aee6211.
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Abstract
The supply of the anticancer drug paclitaxel is limited by its low natural abundance and complex chemical structure. We previously reported the development of a cultured <i>Taxus</i> cambial meristematic cell (CMC) system, where methyl jasmonate enhances paclitaxel production. In this report, we describe a comprehensive analysis to identify potential master regulators of paclitaxel biosynthesis. We report that the combined expression of two myeloblastosis transcription factors can cooperatively activate a subset of paclitaxel biosynthetic genes in stable transgenic CMCs, resulting in a 121-fold increase in paclitaxel and a 364-fold increase in its valuable precursor baccatin III. Our findings provide a powerful approach to enhancing production of this vital compound and can also be applied to other valuable products.