A census of anti-CRISPR proteins reveals AcrIE9 and AcrIE13 as inhibitors of the <i>Escherichia coli</i> K12 type IE CRISPR-Cas system.
basic_science · Level V
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- Record sourced from PubMed, PMID 42258724.
- Also identified by DOI 10.1073/pnas.2529903123.
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Abstract
CRISPR-Cas adaptive immunity systems provide defense against mobile genetic elements and are often countered by diverse anti-CRISPR (Acr) proteins. The type IE CRISPR-Cas of <i><i>Escherichia coli</i></i> K12 has been a model for structural and functional studies and is a part of the species' core genome. However, this system is transcriptionally silent, which has fueled questions about its true biological function. To clarify the role of this system in defense, we carried out a census of Acr proteins in <i>Enterobacterales</i> and identified AcrIE9 as a potent inhibitor of the <i><i>E. coli</i></i> K12 type IE CRISPR-Cas system. While sharing little sequence identity, AcrIE9 proteins from <i><i>Pseudomonas</i></i> and <i><i>Escherichia</i></i> both interact with the Cas7 subunit of the Cascade complex, thus preventing its binding to DNA. We further show that AcrIE9 is genetically linked to AcrIE10, forming the most widespread anti-CRISPR cluster in <i>Enterobacterales</i>; this module often co-occurs with an AcrIE13 protein with an unusual HTH-like architecture.