OrthoFinder: improved phylogenetic orthology inference with enhanced accuracy and scalability.

Emms, David M; Liu, Yi; Belcher, Laurence; Holmes, Jonathan; Kelly, Steven · Nat Methods · 2026

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Abstract

Here we present a major advancement of the OrthoFinder method. This extends OrthoFinder's high-accuracy comparative genomic framework to provide substantially enhanced scalability and accuracy. Specifically, we show that enhanced phylogenetic delineation of orthogroups provides a 7% relative increase in orthogroup inference accuracy. We further demonstrate that a new gene assignment method substantially reduces overall runtime RAM usage without compromising accuracy. The latest version of OrthoFinder is available via GitHub at https://github.com/OrthoFinder/OrthoFinder .