3D pathology-guided microdissection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 42332091.
- Also identified by DOI 10.1038/s41592-026-03141-7.
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Abstract
Traditional micro- and macrodissection techniques enable the extraction of localized regions in thin tissue sections for molecular analysis. Despite the growing use of three-dimensional (3D) microscopy, analogous methods for volumetric microdissection are lacking. Here we have developed a 3D microdissection method based on computer numerical controlled milling integrated with open-top light-sheet microscopy. We demonstrate the ability to study tumor evolution along convoluted 3D branching architectures, which is inaccessible to two-dimensional methods.