Gas-phase unfolding assay rapidly predicts structure-function relationships in engineered antibodies with tuned flexibilities.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 42373639.
- Also identified by DOI 10.1038/s41467-026-75137-y.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Human (h)IgG2 monoclonal antibodies (mAbs) are potent agonists due, in part, to their ability to undergo disulfide shuffling within their hinge regions. Herein, we describe a rapid, sensitive, collision-induced unfolding (CIU) assay that possesses a predictive relationship between gas-phase protein unfolding and agonism in hIgG2 variants. Furthermore, our results highlight the significance of hinge engineering in tuning mAb structure-function relationships for the development of future biotherapeutics.