Discovery of specific rhizosphere bacteria <i>Rhodanobacter</i> involved in KAI2-mediated drought tolerance in <i>Arabidopsis</i>.

Abstract

The KARRIKIN INSENSITIVE 2 (KAI2) receptor has been reported to contribute to drought tolerance in <i>Arabidopsis</i>. However, the extent to which KAI2's function in drought tolerance depends on soil microbiota remains unclear. This study demonstrates that the rhizosphere microbiome is indispensable for KAI2-mediated drought tolerance. We isolated specific <i>Rhodanobacter</i> sp. and confirmed its role in enhancing drought tolerance in <i>Arabidopsis</i>. Notably, <i>Rhodanobacter</i> sp. was found to specifically secrete the key isoflavone daidzin. We found that daidzin had a similar function with KAI2 agonist, desmethyl-type germinone, and induced interaction between KAI2 and SUPRESSOR OF MORE AXILLARY GROWTH 2 1. Moreover, the exogenous application of daidzin enhanced drought tolerance by modulating the expression of karrikin response and drought-related genes, in a KAI2-dependent manner. Our findings suggest that the rhizosphere microbiome plays a crucial role in facilitating KAI2-mediated drought tolerance in <i>Arabidopsis</i>, with <i>Rhodanobacter</i> sp. contributing through the secretion of daidzin.