An oxygen-glucose co-releasing platform fostering dental pulp regeneration by driving metabolic recovery of stem cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 42385491.
- Also identified by DOI 10.1016/j.biomaterials.2026.124414.
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Abstract
In dental pulp regeneration, the ischemic microenvironment within the root canal severely compromises the survival and function of transplanted human dental pulp stem cells (hDPSCs). Here, we developed a PDMS-based core-shell oxygen-glucose delivery platform (P-C@P-G) that continuously released oxygen and glucose for up to 40 and 29 days, respectively, while minimizing peroxide-associated cytotoxicity through diffusion-controlled regulation without the need for exogenous enzymes. Under oxygen-glucose deprivation (OGD, 0.1% O<sub>2</sub>, glucose-free conditions) conditions, P-C@P-G improved cellular metabolic activity and enhanced hDPSC survival, proliferation, migration, and odontogenic differentiation. Compared with single-substrate supplementation, dual oxygen-glucose delivery produced greater improvements in hDPSC survival and differentiation. Transcriptomic and molecular analyses revealed alterations in TNF-α/NF-κB and Wnt/β-catenin signaling pathways following treatment. In vivo, P-C@P-G promoted pulp-like tissue regeneration, vascularization, and dentin sialophosphoprotein (DSPP) expression, resulting in greater pulp-like tissue formation and vascularization than the control group. Collectively, these findings demonstrated that sustained oxygen-glucose delivery effectively alleviated ischemia-associated metabolic insufficiency and revealed distinct contributions of oxygen and glucose to dental pulp regeneration.