Extracting host-specific developmental signatures from longitudinal microbiome data.

Abstract

Longitudinal microbiome studies provide critical insights into microbial community dynamics and their relation to host health. Tensor decompositions offer a powerful framework for the unsupervised analysis of such data, yielding interpretable low-dimensional temporal patterns. However, existing approaches based on the CANDECOMP/PARAFAC (CP) model assume common temporal dynamics for all subjects and therefore cannot capture subject-specific trajectories. To address this limitation, we introduce a novel analytical framework based on PARAFAC2 to explicitly model subject-specific variations, such as shifts and delays in temporal patterns. Through systematic comparisons on simulated and real-world datasets-including studies of infant gut maturation and dietary interventions-we demonstrate that PARAFAC2 outperforms CP in capturing subject-specific temporal trajectories, and enables the discovery of biologically relevant patterns that are overlooked by CP. Furthermore, we introduce replicability as a robust criterion for selecting the number of model components, ensuring that the extracted patterns are replicable.