Integration of multi-stacked biodegradable PLGA membranes into microfluidic organ-on-a-chip devices for tunable barrier co-culture.

Kim, Minju; Choi, Kanghoon; Park, Kibin; Al-Rabadi, Laith; Chang, Jiyoung; Kim, Jungkyu · Lab Chip · 2026

basic_science · Level V

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Abstract

We integrate multi-stacked, thermally fused near-field electrospun PLGA membranes into a microfluidic organ-on-a-chip device. Layer number controls pore diameter (12-33 μm), porosity, circularity and permeability. The biodegradable, optically transparent membranes support endothelial-stromal co-culture with confirmed barrier integrity (TEER, ZO-1) and spatially resolved tissue compartmentalization.