peakScout - a biologist-friendly tool for bidirectional peak-gene mapping.

Lin, Alexander L; Cartailler, Lana A; Cartailler, Jean-Philippe · Bioinformatics · 2026

other · Level V

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Abstract

Translating genomic peak data into biologically meaningful knowledge typically requires bioinformatics expertise, creating a barrier for non-technical users. We developed peakScout to bridge the gap between peaks, genes and gene annotations, enabling users to quickly focus on biological context and not bioinformatics skills, in a reproducible and robust fashion. peakScout is a command line and web-based program that performs bidirectional mapping between genomic peaks and genes. The peak-to-gene mode identifies which genes are potentially regulated by specific genomic regions, while the gene-to-peak mode reveals which regulatory elements might influence particular genes of interest. An algorithm for nearest-feature detection handles the complex spatial relationships between genomic elements, considering factors like distance constraints and feature overlaps. The web version of peakScout is available at https://vandydata.github.io/peakScout/. The command line version is available at https://github.com/vandydata/peakScout and archived on Zenodo (https://doi.org/10.5281/zenodo.21211605) under the GNU Affero General Public License v3.0. Installation instructions, example datasets, and usage examples are provided in the GitHub repository README file. Supplementary data are available at Bioinformatics online.