A detergent-free method for purifying caveolae membrane from tissue culture cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 7479734.
- Also identified by PMC identifier 40744.
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Abstract
Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.
Medical subject headings
- Cell Membrane
- Organelles
- Skin