Lyophilization-induced reversible changes in the secondary structure of proteins.
basic_science · Level V
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- Record sourced from PubMed, PMID 7479920.
- Also identified by PMC identifier 40552.
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Abstract
Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, as revealed by both the quantitative analysis of the second derivative spectra and the Gaussian curve fitting of the original infrared spectra. Lyophilization substantially increases the beta-sheet content and lowers the alpha-helix content of all proteins. In all but one case, proteins become more ordered upon lyophilization.
Medical subject headings
- Freeze Drying
- Protein Structure, Secondary
- Proteins