Eicosanoid excretion from the contralateral kidney in pigs with complete unilateral ureteral obstruction.

Abstract

Eicosanoid excretion from the contralateral kidney in pigs with unilateral ureteral occlusion (UUO) was examined, and the effects of blockade of angiotensin II synthesis and cyclooxygenase inhibition were investigated. Urine obtained from contralateral kidneys in pigs with UUO and from the right kidney in sham-operated pigs was examined for prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) by radioimmunoassay. Excretion rate of PGE2 increased from 307 +/- 69 pg. per minute to 542 +/- 149 pg. per minute (p < 0.05) during UUO. Indomethacin blocked the synthesis of both PGE2 and TxB2. Administration of an inhibitor of the angiotensin I converting enzyme resulted in a significant (p = 0.02) reduction of PGE2 excretion as well as a decrease in TxB2 excretion which was significantly lower 15 hours after UUO. Blockade of angiotensin II synthesis in the sham-operated pigs did not affect prostanoid excretion from their kidneys. This study demonstrates that the presence of angiotensin is important for a full expression of these prostanoids in the contralateral kidney during UUO.