Quality control of imprint and tissue section DNA ploidy analysis in image analysis systems utilizing cell culture-based control materials.

Abstract

Currently, there are no standard controls available for quality control of DNA ploidy assays in image analysis systems. The authors have developed a system of controls based on cultured human cell lines that can mimic tissue sections and touch preparations. This allows for quality control for the entire process for DNA ploidy analysis from the initial cutting of the paraffin block or production of the touch preparation through fixation, staining, and interactive image analysis. The use of cell line controls rather than normal tissue is advantageous because of cell population homogeneity and the volume of uniform slides that are obtainable. This system, while providing an excellent means of controlling day-to-day performance of DNA ploidy analysis, may also be adapted for use as a means of multi-institutional proficiency testing.