Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF.
basic_science · Level V
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- Record sourced from PubMed, PMID 7644475.
- Also identified by PMC identifier 41207.
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Abstract
We show that the higher plant Arabidopsis thaliana has a serine-arginine-rich (SR) protein family whose members contain a phosphoepitope shared by the animal SR family of splicing factors. In addition, we report the cloning and characterization of a cDNA encoding a higher-plant SR protein from Arabidopsis, SR1, which has striking sequence and structural homology to the human splicing factor SF2/ASF. Similar to SF2/ASF, the plant SR1 protein promotes splice site switching in mammalian nuclear extracts. A novel feature of the Arabidopsis SR protein is a C-terminal domain containing a high concentration of proline, serine, and lysine residues (PSK domain), a composition reminiscent of histones. This domain includes a putative phosphorylation site for the mitotic kinase cyclin/p34cdc2.
Medical subject headings
- Arabidopsis
- Arabidopsis Proteins
- Nuclear Proteins
- Plant Proteins