Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.

Ho, K; Nichols, C G; Lederer, W J; Lytton, J; Vassilev, P M; Kanazirska, M V; Hebert, S C · Nature · 1993

basic_science · Level V

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Abstract

A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

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