Sequence-specific scission of DNA by RNAs linked to a chemical nuclease.

Sigman, D S; Chen, C H; Gorin, M B · Nature · 1993

basic_science · Level V

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Abstract

RNAs linked to the chemical nuclease 1,10-phenanthroline-copper cut double-stranded DNA of complementary sequence. This cleavage reaction is applicable to all sequences and can be used to measure the distance between marker genes in base pairs, map the size of a transcription unit and define positions of chromosomal breakpoints.

Medical subject headings