Peptide mimicry of the meningococcal group C capsular polysaccharide.
other · Level V
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- Record sourced from PubMed, PMID 7732025.
- Also identified by PMC identifier 42094.
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Abstract
Sequence analysis of the variable regions of the heavy and light chains of the anti-idiotypic antibody 6F9, which mimics the meningococcal group C capsular polysaccharide (MCP), was performed. The immunogenic site on 6F9 responsible for inducing an anti-MCP antibody response was determined by means of sequence and computer model analysis of these data. Complementarity-determining region 3 (CDR3) was found to be unique in that the sequence tract YRY was exposed on the surface. A synthetic peptide spanning the CDR3 domain was synthesized and complexed to proteosomes (meningococcal group B outer membrane protein). Immunizations of BALB/c mice with the peptide-proteosome complex resulted in a significant anti-MCP antibody response. Immunized mice were protected against infection with a lethal dose of Neisseria meningitidis serogroup C.
Medical subject headings
- Antibodies, Anti-Idiotypic
- Immunoglobulin Heavy Chains
- Immunoglobulin Light Chains
- Immunoglobulin Variable Region
- Neisseria meningitidis
- Polysaccharides, Bacterial
- Protein Structure, Secondary