Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro.
basic_science · Level V
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Abstract
Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.
Medical subject headings
- DNA Replication
- DNA, Viral
- Homeodomain Proteins
- Proto-Oncogene Proteins c-bcl-2
- Repressor Proteins
- Saccharomyces cerevisiae Proteins
- Simian virus 40