HIV-1/HIV-2 seronegativity in HIV-1 subtype O infected patients.

Loussert-Ajaka, I; Ly, T D; Chaix, M L; Ingrand, D; Saragosti, S; Couroucé, A M; Brun-Vézinet, F; Simon, F · Lancet · 1994

case_series · Level IV

Where this comes from

Abstract

Nine patients with atypical HIV-1 western blot profiles were diagnosed as having HIV-1 subtype O infection. All the patients were living in France; eight originated from Cameroon and one from France. Lymphocyte DNA amplification by the polymerase chain reaction was only positive when HIV-1 subtype O specific primers were used. Preliminary sequence analysis of amplified products and serological reactivity against a specific subtype O synthetic env peptide confirmed HIV-1 subtype O infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially those based on env peptides or on the sandwich format, can be negative in HIV-1 subtype O infection.

Medical subject headings