Evaluation of 13 short tandem repeat loci for use in personal identification applications.
basic_science · Level V
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- Record sourced from PubMed, PMID 7912887.
- Also identified by PMC identifier 1918216.
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Abstract
Personal identification by using DNA typing methodologies has been an issue in the popular and scientific press for several years. We present a PCR-based DNA-typing method using 13 unlinked short tandem repeat (STR) loci. Validation of the loci and methodology has been performed to meet standards set by the forensic community and the accrediting organization for parentage testing. Extensive statistical analysis has addressed the issues surrounding the presentation of "match" statistics. We have found STR loci to provide a rapid, sensitive, and reliable method of DNA typing for parentage testing, forensic identification, and medical diagnostics. Valid statistical analysis is generally simpler than similar analysis of RFLP-VNTR results and provides powerful statistical evidence of the low frequency of random multilocus genotype matching.
Medical subject headings
- DNA
- DNA Fingerprinting
- Forensic Medicine
- Polymorphism, Restriction Fragment Length
- Repetitive Sequences, Nucleic Acid