Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation.
basic_science · Level V
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- Record sourced from PubMed, PMID 7937983.
- Also identified by PMC identifier 45049.
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Abstract
Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features--i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical motifs CG and CXG.
Medical subject headings
- Gene Expression Regulation
- Plants, Toxic
- RNA Processing, Post-Transcriptional
- Nicotiana
- Transcription, Genetic