Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 7938010.
- Also identified by PMC identifier 45082.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle replication of circular plasmid templates. Replication is independent of a HSV-1 origin of replication (oris) or the HSV-1 origin binding protein and is inhibited by the origin binding protein when the plasmid contains oris. Replication is dependent on a complex composed of the HSV-1-encoded DNA polymerase and its processivity enhancing factor (the UL42 protein), ICP8 (the HSV-1-encoded single-strand DNA binding protein), and the HSV-1-encoded helicase-primase. The complex can be purified by size-exclusion and anion-exchange chromatography.
Medical subject headings
- DNA Replication
- DNA, Circular
- DNA, Viral
- Exodeoxyribonucleases
- Herpesvirus 1, Human
- Viral Proteins