Crystal structure of the tyrosine kinase domain of the human insulin receptor.

Hubbard, S R; Wei, L; Ellis, L; Hendrickson, W A · Nature

basic_science · Level V

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Abstract

The X-ray crystal structure of the tyrosine kinase domain of the human insulin receptor has been determined by multiwavelength anomalous diffraction phasing and refined to 2.1 A resolution. The structure reveals the determinants of substrate preference for tyrosine rather than serine or threonine and a novel autoinhibition mechanism whereby one of the tyrosines that is autophosphorylated in response to insulin, Tyr 1,162, is bound in the active site.

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