Acute phase vascular endothelial injury: a comparison of temporary arterial occlusion using an endovascular occlusive balloon catheter versus a temporary aneurysm clip in a pig model.

Abstract

We compared the degree of acute endothelial injury after temporary vessel occlusion using two different occlusion modalities--external clipping and endovascular balloon occlusion. The common carotid and subclavian arteries in eight weanling pigs were temporarily occluded with either a 5 Fr occlusion balloon catheter or a temporary microvascular clip for 0 (control), 5, 10, and 30 minutes. Two animals (eight vessels; four clip and four balloon occluded) were used at each time interval. Segments of each experimental vessel were harvested and analyzed by scanning electron microscopy. Each vessel specimen was graded on the following scale: Grade 1 showed no evidence of injury; Grade 2 showed minimal evidence of endothelial cell injury; Grade 3 showed moderate evidence of endothelial cell injury; Grade 4 showed marked evidence of endothelial cell injury; Grade 5 showed severe endothelial and subendothelial injury. Control vessels showed no evidence of injury. Grade 2 injuries were seen in both clip- and balloon-occluded vessels at 5 minutes. At 10 minutes, focal Grade 2 and 3 injuries were appreciated in the clip group, with diffuse Grade 2 and 3 injuries in the balloon group. After the 30-minute occlusion, the balloon group showed Grade 2, 3, and 4 injuries, whereas the clip group showed entirely Grade 2 injuries. The degree of injury with either occlusion modality was equivalent and worsened with time. However, clip-occluded vessels displayed injury that was confined to an area closely adjacent to the clip site, whereas balloon-occluded vessels demonstrated a more widespread injury centered around the balloon site.