Design, creation, and characterization of a stable, monomeric triosephosphate isomerase.
basic_science · Level V
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- Record sourced from PubMed, PMID 8108439.
- Also identified by PMC identifier 43190.
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Abstract
Protein engineering on trypanosomal triosephosphate isomerase (TIM) converted this oligomeric enzyme into a stable, monomeric protein that is enzymatically active. Wild-type TIM consists of two identical subunits that form a very tight dimer involving interactions of 32 residues of each subunit. By replacing 15 residues of the major interface loop by another 8-residue fragment, a variant was constructed that is a stable and monomeric protein with TIM activity. The length, sequence, and conformation of the designed fragment were suggested by extensive modeling.
Medical subject headings
- Triose-Phosphate Isomerase
- Trypanosoma brucei brucei