Multi-target PCR analysis by capillary electrophoresis and laser-induced fluorescence.
basic_science · Level V
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Abstract
Quantitative analysis of polymerase chain reaction (PCR) amplified HIV-1 DNA or cDNA fragments is attained using an automated system that combines capillary-gel electrophoresis (CGE) for high-efficiency separation and laser-induced fluorescence (LIF) for high-sensitivity detection. This system enables the detection of PCR-amplified multiple target DNA or cDNA in the same tube by a single injection with high precision.
Medical subject headings
- DNA, Viral
- Electrophoresis
- HIV-1
- Polymerase Chain Reaction