Systematic HRAS amplification in ovary-independent mammary tumors: correlation with progressively anaplastic phenotypes.

Aldaz, C M; Gollahon, L S; Chen, A · Cancer Res · 1993

basic_science · Level V

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Abstract

Progression of well differentiated rat mammary adenocarcinomas to very anaplastic phenotypes was found to correlate with a systematic and significant amplification of a mutant HRAS allele. Tumors with high amplification levels of this oncogene were analyzed by chromosomal in situ hybridization; in four of the cases the amplified sequences did not reside at the native chromosome 1 locus but were localized in a novel marker chromosome. The model described has potential as a reproducible system for the study of the chromosomal and cellular mechanisms operative "in vivo" for oncogene amplification.

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