Sequencing using pulsed field and image reconstruction.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 8234263.
- Also identified by PMC identifier 47721.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb.
Medical subject headings
- Base Sequence
- DNA
- DNA, Viral
- Genetic Techniques