Cloning of Nrf1, an NF-E2-related transcription factor, by genetic selection in yeast.
basic_science · Level V
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- Record sourced from PubMed, PMID 8248256.
- Also identified by PMC identifier 47984.
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Abstract
We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.
Medical subject headings
- DNA-Binding Proteins
- Trans-Activators
- Transcription Factors