A cytolysin encoded by Salmonella is required for survival within macrophages.
basic_science · Level V
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- Record sourced from PubMed, PMID 8290552.
- Also identified by PMC identifier 42974.
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Abstract
A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.
Medical subject headings
- Bacterial Proteins
- Bacterial Toxins
- Cytotoxins
- Hemolysin Proteins
- Macrophages, Peritoneal
- Salmonella typhimurium
- Transcription Factors