Effects of prostaglandin E1 on the production of IgM and IgG class anti-dsDNA antibodies in NZB/W F1 mice.

Abstract

To investigate the effects of prostaglandin E1 (PGE1) on IgM and IgG class anti-dsDNA antibody production by young and aged female New Zealand black/white (NZB/W) F1 mouse spleen cells in vitro. Whole cells or B cells from NZB/W F1 mouse spleen cells were cultured with lipopolysaccharide (LPS) in the absence or presence of graded concentrations of PGE1 for 1 to 5 days. After cultures, the supernatants were collected and assayed for released IgM and IgG class anti-dsDNA antibodies by enzyme-linked immunosorbent assay. Young (3-month-old) mouse spleen cells produced similar levels of IgM class anti-dsDNA antibodies, while these cells produced considerably low levels of IgG class anti-dsDNA antibodies compared to aged (6-month-old) mouse spleen cells when stimulated with LPS. PGE1 suppressed the production of IgM class anti-dsDNA antibodies by about 50% at a concentration of 10(-6) M in both young and aged mouse spleen cell cultures. On the other hand, the production of IgG class anti-dsDNA antibodies was resistant to the inhibitory effects of PGE1. Our data suggest that PGE1 is effective in inhibiting the antibody synthesis by B cells precommitted to IgM class anti-dsDNA antibody production, but the production of IgG class anti-dsDNA antibody by memory B cells present in young and aged mice is resistant to the inhibitory effects of PGE1.