The isolation of molecular genetic markers for the identification of sex.
basic_science · Level V
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- Record sourced from PubMed, PMID 8378302.
- Also identified by PMC identifier 47348.
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Abstract
It is difficult to identify sex in many species of eukaryotic organism. This can considerably impede research into their biology. Fortunately, one sex often possesses a unique chromosome termed Y or W. When DNA markers are available for these chromosomes, then sex identification becomes straightforward. We describe a technique that facilitates the isolation of such markers. The procedure makes use of low-stringency PCR to screen randomly selected primers for their ability to amplify sex-specific loci.
Medical subject headings
- Birds
- Sex Determination Analysis