Transcription factor TFIID recognizes DNA sequences downstream of the TATA element in the Hsp70 heat shock gene.
basic_science · Level V
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- Record sourced from PubMed, PMID 8378317.
- Also identified by PMC identifier 47374.
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Abstract
The interaction between the Hsp70 heat shock gene promoter and a Drosophila protein complex which contains the TATA-binding protein depends on sequence-specific interactions located in the region downstream of the transcription start site. Immunopurification of the complex through the use of antibodies against the TATA-binding protein reveals that the complex is transcription factor TFIID. Binding assays with the immunopurified TFIID confirm that sequence-specific contacts are made in the region between nucleotides +18 and +33 relative to the transcription start site. These sequence-specific interactions could play key roles in recognition of TATA-containing and TATA-less promoters.
Medical subject headings
- DNA
- DNA-Binding Proteins
- Drosophila
- Heat-Shock Proteins
- Promoter Regions, Genetic
- TATA Box
- Transcription Factors