Enzyme rate assay for 5-fluorocytosine.
basic_science · Level V
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- Record sourced from PubMed, PMID 8379539.
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Abstract
Modification of an enzyme assay for 5-fluorocytosine, from an end point to a rate analysis, has resulted in a method that can provide results within 10 minutes. The modification also rendered the assay less susceptible to interference from bilirubin and triglycerides. This obviated the need to correct 5-fluorocytosine results for icteric or moderately lipemic samples. The performance history of the enzyme rate method has shown that it is reliable, quick, cost-effective, and suitable for use in a clinical laboratory.
Medical subject headings
- Aminohydrolases
- Biological Assay
- Flucytosine