Identification of a 3'-->5' exonuclease activity associated with human RNA polymerase II.
basic_science · Level V
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- Record sourced from PubMed, PMID 8381534.
- Also identified by PMC identifier 45766.
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Abstract
Human RNA polymerase II is shown to be associated with a 3'-->5' exonuclease activity that removes nucleoside 5'-monophosphates from the 3' end of the transcripts in isolated ternary complexes. This activity is stimulated by SII, a protein that acts as a transcription elongation factor in vitro. In addition, we show that another transcription factor, TFIIF, stimulates a competing pyrophosphorolysis reaction. These findings raise interesting questions about the roles of these activities in vivo, including the possibility that this RNA polymerase may proofread the nascent transcript.
Medical subject headings
- Exoribonucleases
- RNA Polymerase II
- Transcription Factors
- Transcription Factors, General
- Transcription Factors, TFII
- Transcription, Genetic
- Transcriptional Elongation Factors