Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing.
basic_science · Level V
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Abstract
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.
Medical subject headings
- DNA, Viral
- HIV-1
- Sequence Analysis, DNA