In vitro selection of optimal DNA substrates for T4 RNA ligase.
basic_science · Level V
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- Record sourced from PubMed, PMID 8434020.
- Also identified by PMC identifier 45917.
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Abstract
We have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 RNA ligase. We find that the ensemble of selected sequences ligated about 10 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly, the majority of the selected sequences approximated a well-defined consensus sequence.
Medical subject headings
- Bacteriophage T4
- DNA
- Oligodeoxyribonucleotides
- RNA Ligase (ATP)