Mapping cis-acting defects in promoters of transcriptionally silent DQA2, DQB2, and DOB genes.
basic_science · Level V
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- Record sourced from PubMed, PMID 8475089.
- Also identified by PMC identifier 46309.
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Abstract
Defects in promoters of the nonexpressed DQA2, DQB2, and DOB genes from the class II major histo-compatibility complex were mapped by placing Z and X boxes of these silent genes into a synthetic DRA promoter. These conserved upstream sequences confer B-cell-specific and gamma-interferon-inducible expression to the DRA gene. Since DRA promoters containing the X box from the DQA2 gene and Z boxes from DQA2, DQB2, and DOB genes were neither expressed constitutively in B cells nor inducible by gamma interferon in fibroblastic cells, these conserved upstream sequences are implicated in the transcriptional defects of these silent genes.
Medical subject headings
- Genes, MHC Class II
- HLA-D Antigens
- HLA-DQ Antigens
- Histocompatibility Antigens Class II
- Promoter Regions, Genetic
- Transcription, Genetic