Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1.
basic_science · Level V
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- Record sourced from PubMed, PMID 8516303.
- Also identified by PMC identifier 46762.
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Abstract
We describe the isolation and characterization of a functional murine platelet/endothelial cell adhesion molecule (PECAM) 1 cDNA clone from a mouse lung library. At the nucleotide level, the coding sequence of murine PECAM-1 is 73% identical to human PECAM-1, and at the amino acid level, the sequence is 79% homologous to its human counterpart. Southern hybridization reveals that one copy of the gene exists in the mouse genome; Northern hybridization reveals a single mRNA species in mouse lung tissue. COS-7 and mouse L cells transfected with murine PECAM-1 expressed a 130-kDa glycoprotein on their surfaces that reacted with anti-murine PECAM-1 monoclonal antibody and comigrated on SDS/PAGE with human PECAM-1. Stable L-cell transfectants aggregate with each other in a PECAM-dependent, homophilic manner.
Medical subject headings
- Antigens, Differentiation, Myelomonocytic
- Blood Platelets
- Cell Adhesion
- Cell Adhesion Molecules
- Lung
- RNA, Messenger